Pacific blue fluorophore

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Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression.Filter. Mirror. Some common fluorochromes. A. V525. 525/50. 495LP. BV510, Pacific Orange, Zombie Aqua. B. V450. 450/50. —. DAPI, BV421, AF405, Pacific Blue, ...Every racial or ethnic group has specific health concerns. On this page, you'll find links to issues that affect Native Hawaiians and Pacific Islanders. Every racial or ethnic group has specific health concerns. Differences in the health of...

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Violet 405 450/50BV421 Pacific Blue VioBlue V450 SuperBright 436 eFluor 450 DAPI Hoechst BFP Sytox Blue Violet 405 525/50BV510 Pacific Orange V500 VioGreen eFluor 506 AmCyan CFP Violet 405 610/20BV605 eVolve 605 SuperBright 600 Violet 405 660/20BV650 eVolve 655 SuperBright 645 Violet 405 710/50BV711 SuperBright 702 Blue 488 525/50FITC AF488 ...Alexa Fluor 405 63 421 - Green 450150 10,20 34000 525 m Banana m Plum pacific O APC.H7 Alexa Fluor 350 Qdot 565 at 545 Fluor 610 Indo-I Blue Low Ca2An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, (E20G/W37T)LplA, that ligates …Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules, Methods in Molecular Biology. 2022 Jan | book-chapter. doi: 10.1007/978-1-0716-1983-4_28. ... The Pennsylvania Green fluorophore: A hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes, Organic Letters. 2006 Jan ...Pacific Blue™ Annexin V - Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. ... but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to ...Spectrum [Pacific Blue] Pacific Blue is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 455 nm. Other spectra of interest include: iFluor 405, iFluor 440, and iFluor 430. Pacific Blue belongs to the following categories: Amine Reactive Dyes and Probes for Conjugation. Purchase Pacific Blue here.1. View Spectra. Normalized emission curves are plotted into each channel off the ultraviolet, violet, blue, yellow/green, and red lasers of Cytek™ Aurora flow cytometers. You can uncheck and remove lasers as necessary to better match your instrument. To view spectra, simply select a fluorophore from one of the dropdowns and then check or ...Annexin V-Pacific Blue Annexin V-eFluor 450 PO-PRO-1 CellTrace Violet Click-iT Plus EdU Pacific Blue Azurite Cerulean TagBFP ECFP mTurquoise AmCyan VioBlue 510/50 520/35 525/50 530/40 550/40 VL2 512/25 VL2 (V6) 525/50 ... Fluorophore and reagent selection guide for flow cytometry* The excitation laser line represents commonly used lasers that excite the fluorochrome. It does not necessarily reflect the lasers available for each particular instrument. † APC and PE-Cy5 may be used together on instruments with cross-beam compensation. ... Pacific Blue ™ Blue 401 405, 407 ...Vio® Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy developed. by Miltenyi Biotec. VioBlue®, VioGreen™, Vio Bright FITC, PE-Vio® 615, PE-Vio 770, PerCP-Vio 700, Vio 515, Vio Bright 515, APC-Vio 770 are characterized by high fluorescence intensities and low spillover, making them an ideal choice for ...An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates …Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BV421, Pacific Blue™ and BD Horizon™ V450 cannot be used simultaneously. Filter: 431/28 or 450/50Yellow-Green 561 nm. Red 633 nm. View our complete Brightness Index. Our technical service team is available to help with any questions you may have on multicolor flow cytometry. Technical Service. Phone Toll-Free (US & Canada)1-877-273-3103. Phone (International): 1-858-768-5801. Email: Click Here.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.In today’s fast-paced world, time is of the essence. With busy schedules and limited time, it is essential to find ways to streamline our tasks and make things more convenient. This applies not only to our daily activities but also to our t...Apr 6, 2012 · For comparison, Pacific Blue (PB), a single molecule dye with near maximum absorption at 405 nm, has an extinction coefficient of 4.6 × 10 4 M −1 cm −1, a quantum efficiency of 78%, and a longer lifetime of 9 ns. Practically, the brightness of a fluorochrome is proportional to the extinction coefficient and quantum efficiency, and ... Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00CyFlow™ CD3 Pacific Blue™. CD3 (T3, Leu4) is expressed on periPacific Orange and Pacific Blue antibody labeling kits enable attachm In vivo, is an accessory photosynthetic pigment found in blue-green algae: APC-Cy7: Allophycocyanin – Cy7: Tandem conjugate where APC is coupled to a cyanine dye (Cy7) Longest wavelength emission in the family of fluorophores excited by the red laser: A488: Alexa Fluor 488: Incredibly stable, so very popular dye: Spectrum almost identical to ... An example is the blue fluorophore Pacific Blue, which is This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of systems.Not for use in diagnostic or therapeutic procedures. APC-Cy7: US patent 5,714,386. Alexa Fluor® is a registered trademark, and Pacific Blue™ is a trademark of ... Fluorochrome chart FL 450/50 530/30 585/40 630/30 670/40 72

Reagent Clone Fluorochrome Stain Index CD4 RPA-T4 BV421 561 Pacific Blue™ 24 PE 142 CD8 RPA-T8 BV421 782 Pacific Blue™ 29 PE 198 CD127 hIL-7R-M21 BV421 55 PE 14 BV421 is a base polymer dye that brings phycoerythrin (PE) level brightness to the violet laser. The BV421 conjugates are, on average, 10 times brighter than Pacific Blue ...The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations ...Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications. Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers.

Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.Alexa Fluor 647 dye. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high ...…

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Every racial or ethnic group has specific health concerns. On this page, you'll find links to issues that affect Native Hawaiians and Pacific Islanders. Every racial or ethnic group has specific health concerns. Differences in the health of...PB NHS Ester. PB dye (Pacific Blue™) is a bright, blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. The excitation and emission spectrum of PB dye offers minimal overlap with green fluorophore emission spectra, making it an ideal choice for the first violet channel. Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...

Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications. Brilliant Violet dyes, including Brilliant Violet 421 (BV421), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels. BV421 is excited by the 405 nm violet laser and emits at ... This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of systems.

The difference between Central and Pacific Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ... An Improved Synthesis of the Pacific Blue FluoropWe report a new method to quantify the affinity of small molecule Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: …Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00 Yellow-Green 561 nm. Red 633 nm. View our compl 19 нояб. 2020 г. ... ... Pacific Blue and eFluor450 can be alternatives to BV421 but they are dimmer fluorophores. Fluorophore brightness is also dependent on ... Alexa Fluor dyes—Emission spectra. Figure 1. EmissionInvitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescFixable Blue DAPI SYTOX™ Blue Hoechst 33342 ... C Find reagents for flow cytometry Select fluorophore ... Pacific Blue BD Horizon V450: Azurite Cerulean eBFP eCFP mTurquoise Sirius: 512/25: VL2: eFluor 506 VioGreen Brilliant Violet 510 Pacific Green BD Horizon V500: T-Sapphire CFP vGFP: 603/48: VL3: Super Bright 600 Qdot 605 Pacific OrangePacific Blue 455 V4. BV480. 480 BV510 510. eFluor506. 510. V500. 510 V8 Pacific Orange 550 V9. BV570. 570. Super Bright 600. 600 BV605 605 V11 BV650 650. Super Bright 702. 702 BV711 711 V14. BV750. 750 ... 830nm) of eac h fluorochrome providing a unique signature for each dye, this allows combinations that conventional cytometers cannot us … Pacific Blue dye. Fluorescence SpectraViewer. Invitrogen Molecular Pr Yellow-Green 561 nm. Red 633 nm. View our complete Brightness Index. Our technical service team is available to help with any questions you may have on multicolor flow cytometry. Technical Service. Phone Toll-Free (US & Canada)1-877-273-3103. Phone (International): 1-858-768-5801. Email: Click Here. Invitrogen Molecular Probes Pacific Blue dye is a bright blu[Quantification of Small Molecule-ProteinIf you look at a map of the British Columbian coastline, you’ll see An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, (E20G/W37T)LplA, that ligates …